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| 高性能ESI-Ion
Trap MSn システム |
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今日、研究者には、LC/MSnの明確な要求があります。高感度と高速スキャンがHPLCの各ピークから得られる情報を最大限に引き出します。
データ解釈と管理を簡素化する賢いソフトウェアツール。
増加するサンプル数とスループットの直線的な作業フロー。
傑出したスキャンパワー、感度、および便利さでどうこれらの必要性に答えるかを見てください。
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| Industry-leading scan power |
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| Explore the high performance of our 3rd generation benchtop ion
trap. Perform rapid sample screening, as well as detailed structural and quantitative
investigations. |
| Applications-driven software
suite |
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| Utilize powerful software tools for quick and intuitive method
setup, data interpretation and reporting of results. |
| An analytical platform tailored
to your needs |
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| Benefit from the esquire‘s smooth integration with a broad variety
of LC systems, sample preparation modules, and API sources. |
■Chromatography
Choices
The most widely used LC systems, autosamplers and fraction collectors
are now fully integrated with the esquire6000: The Compass software enables complete
and intuitive LC/MS method setup and system control.
The esquire6000 can be coupled to the Capillary Electrophoresis systems and is
part of the world‘s first fully-integrated LC-NMR/MS system introduced by Bruker
Biospin and Bruker Daltonics. |
■Ion
Sources
The esquire6000 uses the Agilent electrospray (ESI) ion source,
which provides outstanding robustness, sensitivity and reproducibility over a
wide range of LC operating conditions.
The orthogonal sprayer design speeds up method development while minimizing chemical
noise, regardless of the separation conditions. The rugged design with fixed sprayer
position prevents contamination and thus extends the maintenance cycle time.
Enhanced sensivitity for all applications down to the very low pg limit is possible.
Tuning has become easier and largely uncritical across a wide m/z range.
Switching between all sources is fast and easy, due to the hinged swingout spray
chamber design. A whole varitey of API sources covers the widest range of LC/MS
applications, including various low-flow options. |
■The
Ion Trap
| Superior combination of mass
range, scan speed and resolution. |
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| The ion trap continues to offer the unrivaled standard mass range
up to m/z 3000. Monoisotopic resolution throughout this standard mass range gives
you the full picture at once. The extended mass range reaches up to m/z 6000.
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| Fast scanning |
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| Ultrafast scan cycles for both MS and MS/MS account for an extremely
high duty cycle. More than 100 mass scans can be captured across a typical LC
peak. This fast scanning also improves the dynamic range and reduces measurement
variance. |
| MSn performance |
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| Fragmentation is supported up to the MS11 stage, allowing for detailed
investigation of target compounds. Monoisotopic isolation is ensured throughout
the standard mass range. |
| Sensitivity |
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| The highly efficient ion transfer and trapping, as well as short
scan times contribute to the truly superior sensitivity in the low pg range. |
| Multiple stages of structural
information |
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Multiple fragmentation experiments up to MS11 help to gather structural
information on ingredients of natural products, drugs, food and other samples.
Because of the single-step dissociation in an ion trap, fragmentation pathways
can be elucidated easily by MSn. An optional Wide-band Fragmentation™ across a
m/z range of up to 300 u ensures the simultaneous fragmentation of the precursor
and ions which are derived from the pure loss of water or amine groups if those
occur dominantly.
Fully automated ion-tree experiments and data-dependent scans help gaining structural
information very quickly. In order to extract only the most relevant information,
intelligent AutoMS/MS options can be used for MS/MS precursor ion selection and
fragmentation. |
■Applications
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Automated Protein Identification |
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In-Depth Protein Characterization |
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Intelligent data acquisition
in Proteomics |
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Structural Confirmation |
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Quantitation from complex
samples |
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Metabolite Screening |
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■Technical
specifications
| Proven esquire technology
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Unparalleled combination
of resolution, scan speed, and mass range in MS and MSn. |
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Superior mass accuracy. |
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Low pg MS and MS/MS sensitivity.
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Fast Polarity Switching for
on-the-fly acquisition of positive and negative ions. |
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SmartFrag™ creates reproducible
MS/MS spectra for successful MS/MS library search. |
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| Scan speed and resolution (MS and MS/MS) |
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| Scan Mode
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Resolution
FWHM (u) |
m/z
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u/sec
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| Standard |
0.6 |
50-3000 |
13.000 |
| Enhanced |
0.45 |
50-3000 |
5.500 |
| Maximum Res. |
0.35 |
50-3000 |
1.650 |
| Extended |
2-4 |
200-6000 |
27.000 |
| Scan modes |
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Full scan MS and MS/MS in
all scan modes for sensitive and fast analysis of unknown compounds. |
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Neutral loss scans e.g. for
determination of protein modifications. |
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Multiple reaction monitoring
(MRM) with MS/MS and MS3. |
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Manual MSn up to MS11 in
all scan modes. |
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AutoMSn tree experiments
with up to 30 MS precursor ions and 5 fragment ions per stage. |
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Triple Play experiments without
time consuming “charge-state determination-scan“. |
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| Data-dependent experiments
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All types of data-dependent
experiments, including preferred mass list for automated feedback experiments
from MetaboliteTools and PROTEINEER. |
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Active Exclusion |
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Passive Exclusion |
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Maximum Resolution Scan |
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Triple Play experiments without
time consuming "charge-state determination-scan" |
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Preferred mass list |
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Preferred charge state |
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| Chromatography Choices |
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| Integrated LC-MS control provided for: |
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Agilent 1100 HPLC series,
Capillary LC, nanoLC |
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Waters Alliance HPLC series,
CapLC, PDA |
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LC Packings nanoLC systems
(1D and 2D) |
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Gilson 215 Autosampler/Fraction
Collector |
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Spark Endurance Autosampler |
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CTC Analytics HTS PAL Autosampler
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| Source Options |
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ESI - Orthogonal electrospray
ionization source |
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APCI - Orthogonal atmospheric
pressure chemical ionization source |
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Online NanoElectrospray -
for capillary LC flow down to 50 nL/min |
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Offline NanoElectrospray
- consumption of 1-2 uL per sample at a flow rate of ca. 25 nL/min |
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AP-MALDI - Atmospheric pressure
MALDI source |
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APPI - Atmospheric pressure
photo ionization source |
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Smart CE-MS coupling with
grounded needle |
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| COMPASS - Software Features |
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Integrated LC-MS control and data processing, including HyStar™, esquireControl™,
DataAnalysis™, QuantAnalysis™, LibrarySearch™ modules.
Software options: |
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MetaboliteTools™ software
for metabolite identification |
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BioTools™/RapiDeNovo™ software
for protein data interpretation |
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ProteinScape™ database system
for proteome project management |
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ACD Labs MS Manager |
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Compass Security Pack for
work in regulated environment |
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■Related
Products
■Further
Reading
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